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Integrated Proteomics Applications rawconverter
Rawconverter, supplied by Integrated Proteomics Applications, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rawconverter/rawconverter/bio_rxiv__64898__2026__02__01__703113-398-12-20
Average 86 stars, based on 1 article reviews
rawconverter - by Bioz Stars, 2026-09
86/100 stars

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Related Articles

Quantitation Assay:

Article Title: Comparison of CRISPR Genomic Tagging for Affinity Purification and Endogenous Immunoprecipitation Coupled with Quantitative Mass Spectrometry To Identify the Dynamic AMPKα2 Interactome.
Article Snippet: .. Raw files were again parsed into MS1 and MS2 spectra using RawConverter.33 Protein Identification and Quantitation Protein identification, quantification, and analysis were done with Integrated Proteomics Pipeline - IP2 (Integrated Proteomics Applications, Inc., www.integratedproteomics. com/) using ProLuCID,34 DTASelect2,35 Census,36 and QuantCompare.30 MS/MS spectra were searched with ProLuCID, against the human UniprotKB database, released on March 25, 2014 (http://www.uniprot.org/downloads) concatenated to a decoy database in which the sequence for each entry in the original database was reversed. ..

Tandem Mass Spectroscopy:

Article Title: Comparison of CRISPR Genomic Tagging for Affinity Purification and Endogenous Immunoprecipitation Coupled with Quantitative Mass Spectrometry To Identify the Dynamic AMPKα2 Interactome.
Article Snippet: .. Raw files were again parsed into MS1 and MS2 spectra using RawConverter.33 Protein Identification and Quantitation Protein identification, quantification, and analysis were done with Integrated Proteomics Pipeline - IP2 (Integrated Proteomics Applications, Inc., www.integratedproteomics. com/) using ProLuCID,34 DTASelect2,35 Census,36 and QuantCompare.30 MS/MS spectra were searched with ProLuCID, against the human UniprotKB database, released on March 25, 2014 (http://www.uniprot.org/downloads) concatenated to a decoy database in which the sequence for each entry in the original database was reversed. ..

Sequencing:

Article Title: Comparison of CRISPR Genomic Tagging for Affinity Purification and Endogenous Immunoprecipitation Coupled with Quantitative Mass Spectrometry To Identify the Dynamic AMPKα2 Interactome.
Article Snippet: .. Raw files were again parsed into MS1 and MS2 spectra using RawConverter.33 Protein Identification and Quantitation Protein identification, quantification, and analysis were done with Integrated Proteomics Pipeline - IP2 (Integrated Proteomics Applications, Inc., www.integratedproteomics. com/) using ProLuCID,34 DTASelect2,35 Census,36 and QuantCompare.30 MS/MS spectra were searched with ProLuCID, against the human UniprotKB database, released on March 25, 2014 (http://www.uniprot.org/downloads) concatenated to a decoy database in which the sequence for each entry in the original database was reversed. ..

Software:

Article Title: Isoform-selective activity-based profiling of ERK signaling †Electronic supplementary information (ESI) available: Experimental Methods and Supplementary Figures. See DOI: 10.1039/c8sc00043c
Article Snippet: The eluted peptides were electrosprayed into an Orbitrap Q Exactive Plus mass spectrometer (Thermo Scientific), which was operated with a top 10 data-dependent acquisition method that consisted of one full MS1 scan (375 - 1,500 m/z) followed by 10 MS2 scans of the most abundant ions recorded in the MS1 scan. .. Data analysis was accomplished using the IP2 (Integrated Proteomics Applications) software package, in which RawConverter was used to generate searchable MS1 and MS2 data from the .raw file followed by using the ProLuCID algorithm (publicly available at http://fields.scripps.edu/downloads.php) to search the data against a modified human protein database (UniProt human protein database with rat DGKs, angiotensin I and vasoactive intestinal peptide standards; 40,660 proteins) with the following parameters: static carbamidomethyl modification of cysteine (+57.0142 Da), differential modifications corresponding oxidized methionine (+15.9949 Da) and desthiobiotin-labeled lysine residues (+196.1212 Da), added masses of the SILAC “heavy”-labeled amino acids (+10.0083 Da for R, +8.0142 Da for K), and trypsin enzyme specificity with 2 missed cleavages. ..

Modification:

Article Title: Isoform-selective activity-based profiling of ERK signaling †Electronic supplementary information (ESI) available: Experimental Methods and Supplementary Figures. See DOI: 10.1039/c8sc00043c
Article Snippet: The eluted peptides were electrosprayed into an Orbitrap Q Exactive Plus mass spectrometer (Thermo Scientific), which was operated with a top 10 data-dependent acquisition method that consisted of one full MS1 scan (375 - 1,500 m/z) followed by 10 MS2 scans of the most abundant ions recorded in the MS1 scan. .. Data analysis was accomplished using the IP2 (Integrated Proteomics Applications) software package, in which RawConverter was used to generate searchable MS1 and MS2 data from the .raw file followed by using the ProLuCID algorithm (publicly available at http://fields.scripps.edu/downloads.php) to search the data against a modified human protein database (UniProt human protein database with rat DGKs, angiotensin I and vasoactive intestinal peptide standards; 40,660 proteins) with the following parameters: static carbamidomethyl modification of cysteine (+57.0142 Da), differential modifications corresponding oxidized methionine (+15.9949 Da) and desthiobiotin-labeled lysine residues (+196.1212 Da), added masses of the SILAC “heavy”-labeled amino acids (+10.0083 Da for R, +8.0142 Da for K), and trypsin enzyme specificity with 2 missed cleavages. ..

Multiplex sample analysis:

Article Title: Isoform-selective activity-based profiling of ERK signaling †Electronic supplementary information (ESI) available: Experimental Methods and Supplementary Figures. See DOI: 10.1039/c8sc00043c
Article Snippet: The eluted peptides were electrosprayed into an Orbitrap Q Exactive Plus mass spectrometer (Thermo Scientific), which was operated with a top 10 data-dependent acquisition method that consisted of one full MS1 scan (375 - 1,500 m/z) followed by 10 MS2 scans of the most abundant ions recorded in the MS1 scan. .. Data analysis was accomplished using the IP2 (Integrated Proteomics Applications) software package, in which RawConverter was used to generate searchable MS1 and MS2 data from the .raw file followed by using the ProLuCID algorithm (publicly available at http://fields.scripps.edu/downloads.php) to search the data against a modified human protein database (UniProt human protein database with rat DGKs, angiotensin I and vasoactive intestinal peptide standards; 40,660 proteins) with the following parameters: static carbamidomethyl modification of cysteine (+57.0142 Da), differential modifications corresponding oxidized methionine (+15.9949 Da) and desthiobiotin-labeled lysine residues (+196.1212 Da), added masses of the SILAC “heavy”-labeled amino acids (+10.0083 Da for R, +8.0142 Da for K), and trypsin enzyme specificity with 2 missed cleavages. ..

Article Title: Compositions and uses thereof
Article Snippet: .. 30 DTASelect 2.0 using the —mass, —modstat, and —trypstat options with a 1% peptide FDR. mzIdent files corresponding to searches were generated in IP2-Integrated Proteomics Pipeline, mzXML spectra data was extracted from the raw file using RawConverter, and uploaded into Skyline-daily (Schilling et al., 2012) to determine SILAC ratios (SR) of light/heavy (vehicle/compound treated) peptides. ..

Article Title: The Ligand Binding Landscape of Diacylglycerol Kinases
Article Snippet: Data analysis was accomplished using the IP2 (Integrated Proteomics Applications) software package, in which RawConverter was used to generate searchable MS1 and MS2 data from the .raw file followed by using the ProLuCID algorithm to search the data against a modified human protein database (UniProt human protein database with rat DGKs, angiotensin I and vasoactive intestinal peptide standards; 40,660 proteins) with the following parameters: static carbamidomethyl modification of cysteine (+57.0142 Da), differential modifications of oxidized methionine (+15.9949 Da) and desthiobiotin-labeled lysine residues (+196.1212 Da), added masses of the SILAC “heavy”-labeled amino acids (+10.0083 Da for R, +8.0142 Da for K), and trypsin enzyme specificity with 2 missed cleavages. .. The resulting MS2 spectra matches were assembled into protein identifications and filtered using DTA Select 2.0 using the --mass, --modstat, and --trypstat options with a 1% peptide FDR. mzIdent files corresponding to searches were generated in IP2-Integrated Proteomics Pipeline, mzXML spectra data was extracted from the raw file using RawConverter, and uploaded into Skyline-daily ( Schilling et al., 2012 ) to determine SILAC ratios ( SR ) of light/heavy (vehicle/compound treated) peptides. ..

Generated:

Article Title: Compositions and uses thereof
Article Snippet: .. 30 DTASelect 2.0 using the —mass, —modstat, and —trypstat options with a 1% peptide FDR. mzIdent files corresponding to searches were generated in IP2-Integrated Proteomics Pipeline, mzXML spectra data was extracted from the raw file using RawConverter, and uploaded into Skyline-daily (Schilling et al., 2012) to determine SILAC ratios (SR) of light/heavy (vehicle/compound treated) peptides. ..

Article Title: The Ligand Binding Landscape of Diacylglycerol Kinases
Article Snippet: Data analysis was accomplished using the IP2 (Integrated Proteomics Applications) software package, in which RawConverter was used to generate searchable MS1 and MS2 data from the .raw file followed by using the ProLuCID algorithm to search the data against a modified human protein database (UniProt human protein database with rat DGKs, angiotensin I and vasoactive intestinal peptide standards; 40,660 proteins) with the following parameters: static carbamidomethyl modification of cysteine (+57.0142 Da), differential modifications of oxidized methionine (+15.9949 Da) and desthiobiotin-labeled lysine residues (+196.1212 Da), added masses of the SILAC “heavy”-labeled amino acids (+10.0083 Da for R, +8.0142 Da for K), and trypsin enzyme specificity with 2 missed cleavages. .. The resulting MS2 spectra matches were assembled into protein identifications and filtered using DTA Select 2.0 using the --mass, --modstat, and --trypstat options with a 1% peptide FDR. mzIdent files corresponding to searches were generated in IP2-Integrated Proteomics Pipeline, mzXML spectra data was extracted from the raw file using RawConverter, and uploaded into Skyline-daily ( Schilling et al., 2012 ) to determine SILAC ratios ( SR ) of light/heavy (vehicle/compound treated) peptides. ..

Targeted Proteomics:

Article Title: Compositions and uses thereof
Article Snippet: .. 30 DTASelect 2.0 using the —mass, —modstat, and —trypstat options with a 1% peptide FDR. mzIdent files corresponding to searches were generated in IP2-Integrated Proteomics Pipeline, mzXML spectra data was extracted from the raw file using RawConverter, and uploaded into Skyline-daily (Schilling et al., 2012) to determine SILAC ratios (SR) of light/heavy (vehicle/compound treated) peptides. ..

Article Title: The Ligand Binding Landscape of Diacylglycerol Kinases
Article Snippet: Data analysis was accomplished using the IP2 (Integrated Proteomics Applications) software package, in which RawConverter was used to generate searchable MS1 and MS2 data from the .raw file followed by using the ProLuCID algorithm to search the data against a modified human protein database (UniProt human protein database with rat DGKs, angiotensin I and vasoactive intestinal peptide standards; 40,660 proteins) with the following parameters: static carbamidomethyl modification of cysteine (+57.0142 Da), differential modifications of oxidized methionine (+15.9949 Da) and desthiobiotin-labeled lysine residues (+196.1212 Da), added masses of the SILAC “heavy”-labeled amino acids (+10.0083 Da for R, +8.0142 Da for K), and trypsin enzyme specificity with 2 missed cleavages. .. The resulting MS2 spectra matches were assembled into protein identifications and filtered using DTA Select 2.0 using the --mass, --modstat, and --trypstat options with a 1% peptide FDR. mzIdent files corresponding to searches were generated in IP2-Integrated Proteomics Pipeline, mzXML spectra data was extracted from the raw file using RawConverter, and uploaded into Skyline-daily ( Schilling et al., 2012 ) to determine SILAC ratios ( SR ) of light/heavy (vehicle/compound treated) peptides. ..



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